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KMID : 1036720170500050415
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Journal of Nutrition and Health
2017 Volume.50 No. 5 p.415 ~ p.425
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Neuroprotective effects of Momordica charantia extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SK-N-MC cells
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Kim Kkot-Byeol
Lee Sun-Ah
Heo Jae-Hyeok
Kim Jung-Hee
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Abstract
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Purpose: Many studies have suggested that neuronal cells protect against oxidative stress-induced apoptotic cell death by
polyphenolic compounds. We investigated the neuroprotective effects and the mechanism of action of Momordica charantia ethanol extract (MCE) against H2O2-induced cell death of human neuroblastoma SK-N-MC cells.
Methods: The antioxidant activity of MCE was measured by the quantity of total phenolic acid compounds (TPC), quantity of total flavonoid compounds (TFC), and 2,2-Diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging activity. Cytotoxicity and cell viability were determined by CCK-8 assay. The formation of reactive oxygen species (ROS) was measured using 2,7-dichlorofluorescein diacetate (DCF-DA) assay. Antioxidant enzyme (SOD-1,2 and GPx-1) expression was determined by real-time PCR. Mitogen-activated protein kinases (MAPK) pathway and apoptosis signal expression was measured by
Western blotting.
Results: The TPC and TFC quantities of MCE were 28.51 mg gallic acid equivalents/extract g and 3.95 mg catechin equivalents/extract g, respectively. The IC50 value for DPPH radical scavenging activity was 506.95 μg/ml for MCE. Pre-treatment with MCE showed protective effects against H2O2-induced cell death and inhibited ROS generation by
oxidative stress. SOD-1,2 and GPx-1 mRNA expression was recovered by pre-treatment with MCE compared with the
presence of H2O2. Pre-treatment with MCE inhibited phosphorylation of p38 and the JNK pathway and down-regulated
cleaved caspase-3 and cleaved PARP by H2O2.
Conclusion: The neuroprotective effects of MCE in terms of recovery of antioxidant enzyme gene expression, down-regulation of MAPK pathways, and inhibition apoptosis is associated with reduced oxidative stress in SK-N-MC cells.
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KEYWORD
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Momordica charantia ethanol extract (MCE), antioxidant, MAPK pathway, apoptosis, SK-N-MC
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